|
KMID : 0387720090200020105
|
|
Korean Journal of Blood Transfusion
2009 Volume.20 No. 2 p.105 ~ p.112
|
|
|
A Study to Verify the Stability of Currently Stored Blood Donor Samples (A First Year Study)
|
|
Oh Deok-Ja
Youn Kyoung-Won
Lee Jae-Sook
Kang Jae-Won
Seo Hye-Jun
Yoon Man-Jung
Woo Kyung-Nam
Yoon Ji-Yeon
Suh In-Bum
Kim Jae-Seok
Park Hae-Joon
Ha Mi-Na
Kwon So-Young
Hwang Yoo-Sung
|
|
|
Abstract
|
|
|
Background: The Korean Red Cross (KRC) has stored blood donor samples for 10 years under ?20oC since 2004. These samples have been used for investigating transfusion related infections and for Look-back studies. We designed an experimental scheme to verify the stability of stored blood samples.
Methods: We collected and prepared samples such as blood donor samples (HBV, HCV, HIV nucleic acid positive; n=90), the HIV infected patient samples (n=20), the WHO nucleic acid international standards serologic positive samples (HBsAg, anti-HCV, anti-HIV; n=120) and the negative samples (n=20). The samples were aliquoted in cryo tubes with volumes of 0.5∼5 mL and they were stored at ?20∼?30oC and ?70∼?80oC. We used enzyme immunoassay, chemiluminescence immunoassay and quantitative PCR for the base line and the follow up studies. The linear mixed statistical model using SAS 9.1 for windows was used for statistical analysis.
Results: The results of the baseline test of the stored samples showed a variable range of viral load (101∼107 IU/mL or copies/mL) and optical density (S/CO 3.0∼500). The results of the stored samples after 6 month (n=82) did not show any significant differences compared to the baseline data for the viral loads (P＞0.05) and the qualitative serologic tests.
Conclusion: We established an experimental scheme to verify the stability of the stored blood donor samples. From now on, the stability of the stored samples is going to be monitored by every 6 month for 10 years.
|
|
|
KEYWORD
|
|
|
Blood donor archive, Stability of the stored samples, Quantitative PCR, Serologic test
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|